Coding

Part:BBa_M36106:Design

Designed by: Andee Wallace, Taylor Nguyen, Chad Viergever   Group: Stanford BIOE44 - S11   (2011-05-03)

HY5 3 BS Promoter w/ Gemini, LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 926


Design Notes

Using 3 binding sites in the promoter to test variations. We anticipate HY5 will bind to these sites, inhibiting DNA polymerase from binding and starting transcription.

Source

M36100 (3 binding site promoter), M36709 actuator

References

HY5 bindind sites: http://www.uniprot.org/uniprot/O24646